159,284 results

1. pEGFP-C1-G3BP1-DeltaRBP

   Plasmid

   #135999

   Purpose

   G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-term of G3BP1 Deleted for Delta RBP

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIG-w

   Plasmid

   #12282

   Purpose

   a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

   Depositor

   Luk Parijs

   Article

   Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ADAM10-HA

   Plasmid

   #65106

   Purpose

   Expression of HA tagged ADAM10 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB5320

   Plasmid

   #204830

   Purpose

   An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW2 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3basic luciferase and renilla_polyA

   Plasmid

   #128046

   Purpose

   pGL3 basic luciferase reporter with co-expression of renilla luciferase

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. BRD4

   Plasmid

   #38943

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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