Addgene

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Bax gRNA#3

Plasmid

#171829

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Cdc42

Plasmid

#163606

Purpose

Expresses Cdc42 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Cdc42 (Cdc42 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGMR-WhiteA2

Plasmid

#165874

Purpose

Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-ble

Plasmid

#165880

Purpose

Basic Phleomycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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KG#471

Plasmid

#110935

Purpose

Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

Inserts

mig-13 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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ELK1 A box-NLS pcDNA3.1/HisC

Plasmid

#121096

Purpose

Expression of the A box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Tags

6XHis-Omni-xpress

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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ELK1 B box-NLS pcDNA3.1/HisC

Plasmid

#121095

Purpose

Expression of the B box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Tags

6XHis-Omni-xpress

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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KG#493

Plasmid

#110932

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#94

Plasmid

#110931

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM2112-5p

Plasmid

#84109

Purpose

target miRNA2112-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2112-5p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-OCT-HA-eGFP

Plasmid

#67484

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV3-OCT-HA-eGFP

Plasmid

#67482

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S408A

Plasmid

#44109

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S408A (TRAF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 408 to Alanine

Promoter

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
Dom34 E261K

Plasmid

#19109

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Use

Tags

C tag

Expression

Bacterial

Mutation

E261K

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCB578

Plasmid

#141095

Purpose

E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Inserts

Cas9 and tracrRNA
repeat-spacer-repeat array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
p10xUAS-luc2

Plasmid

#173006

Purpose

firefly luciferase reporter vector with 10x clustered UAS binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

10xUAS-luc2

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge