We narrowed to 8,358 results for: BLI;

1. pcDNA3.1-hSyncytin-1(C186A)

   Plasmid

   #182450

   Purpose

   hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C186A

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG Retro EGFP Puro

   Plasmid

   #234463

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAG Retro EGFP Hygro

   Plasmid

   #234462

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCWU50

   Plasmid

   #232318

   Purpose

   A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   fdx

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

   Plasmid

   #221449

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

   Plasmid

   #221450

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

   Plasmid

   #221451

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid

    #218970

    Purpose

    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

    Depositor

    Benjamin Glick

    Insert

    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    moxGFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUSE-URA3-Sec7-msYFP2

    Plasmid

    #218969

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

    Depositor

    Benjamin Glick

    Insert

    SEC7-msYFP2 (SEC7 Budding Yeast)
    

    Use

    Tags

    msYFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiGuideSLC35C1-neo

    Plasmid

    #220868

    Purpose

    encodes CRISPR sgRNA targeting human SLC3C1

    Depositor

    Jennifer Kohler

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. icLCT

    Plasmid

    #223179

    Purpose

    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)

    Depositor

    Andreas Stuermer

    Insert

    cytosolic intestinal lactase protein fragment (Lct Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pXR004_puroR

    Plasmid

    #219820

    Purpose

    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCC1R

    Plasmid

    #213497

    Purpose

    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MUT_S1-H1

    Plasmid

    #218394

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MUT_S7-H1

    Plasmid

    #218396

    Purpose

    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-SYN-eGFP-oPRE

    Plasmid

    #218681

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid

    #218194

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3

    Depositor

    Florence Pojer

    Insert

    EndoF3
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-329

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-29b(+)_His-CBD-EmEndoF1

    Plasmid

    #218195

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1

    Depositor

    Florence Pojer

    Insert

    EndoF1
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 51-339

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG426-GAL-Prepro-3x-Adan

    Plasmid

    #181733

    Purpose

    Galactose inducible expression of Prepro-3x-Adan

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Adan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG416-GAL-RNQ1

    Plasmid

    #181706

    Purpose

    Galactose inducible expression of RNQ1

    Depositor

    Alejandro Chavez

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #211337

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3075-Left TALE-G1397-N-DddA11-mCherry
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7CFE1_EVA71_VP12A

    Plasmid

    #212977

    Purpose

    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7CFE1_EVD68_VP12A

    Plasmid

    #212984

    Purpose

    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7CFE1_EVD68_3ABC

    Plasmid

    #212979

    Purpose

    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABC
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pT7CFE1_EVD68_3ABCD

    Plasmid

    #212981

    Purpose

    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABCD
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC-ODN

    Plasmid

    #208642

    Purpose

    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Long single strand dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR3cDual-Wnk

    Plasmid

    #203976

    Purpose

    Enrty clone for Gateway cloning

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. DV.7.1

    Plasmid

    #208584

    Purpose

    Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant DV.7.1 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HA Display H3_A/Hong Kong/1/1968 Part 4-DO

    Plasmid

    #206518

    Purpose

    CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells

    Depositor

    IIya Finkelstein

    Type

    Empty backbone

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HA Display H3_A/Hong Kong/1/1968 Part 1-DO

    Plasmid

    #206515

    Purpose

    CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells

    Depositor

    IIya Finkelstein

    Type

    Empty backbone

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG416GAL-Bat-SL-CoVZC45_PLP

    Plasmid

    #201939

    Purpose

    Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    Bat-SL-CoVZC45 PLP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG416GAL10-Bat-SL-CoVZC45_3CL

    Plasmid

    #201944

    Purpose

    Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    Bat-SL-CoVZC45 3CL protease
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG416GAL10-DUGV_OTU

    Plasmid

    #203463

    Purpose

    Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    DUGV OTU domain of L protein (DUGVsLgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge