Addgene

We narrowed to 7,516 results for: Ski;

Showing: 1741 - 1760 of 7516 results

Results per page:

Sort results by:

pEF5/FRT/DEST-3xHA/mGli2 P5-6A

Plasmid

#51253

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
Gal4-VP16-HSF1Δ∼PRD

Plasmid

#71727

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Human, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-SUMO-Blk-SH2-AviTag

Plasmid

#214204

Purpose

Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Science. 2025, 34:e70317.

Insert

Blk kinase SH2 domain (BLK Human)

Use

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-DR30(SapI)-CMV-intron-MCS-pA

Plasmid

#192496

Purpose

To express CasRX gRNA and contains MCS to insert coding region of interest

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Tags

Expression

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

Plasmid

#233038

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233034

Purpose

To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d and mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233032

Purpose

To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx and mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX RBM42-HA

Plasmid

#229500

Purpose

Lentiviral expression vector to express RBM42 isoform 1 with HA-tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

RBM42 (RBM42 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB9P3

Plasmid

#210034

Purpose

Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-FKBP-Halo-Dync1h1motor

Plasmid

#191333

Purpose

Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-FKBP-Dync1h1motor

Plasmid

#191332

Purpose

Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-MAVS-Gln93

Plasmid

#158629

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

E93Q

Promoter

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-MAVS-Ala148

Plasmid

#158630

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q148A

Promoter

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-D4 miR30-AGO1 ts2

Plasmid

#115851

Purpose

AGO1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO1 shRNA (AGO1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-NRASQ61K/E37A-IRES-Puro

Plasmid

#120572

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge