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pMXs-IP GFP-Omp25

Plasmid

#38249

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

plasmid contains AA109-145 of rat Omp25/Synj2bp

Promoter

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Expression

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELK3

Plasmid

#167750

Purpose

Expresses ELK3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELK3 (ELK3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔPRYSPRY

Plasmid

#105521

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-FNTA

Plasmid

#86859

Purpose

To express FNTA with a Venus tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Tags

Expression

Mammalian

Mutation

Promoter

Tet responsive element

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-Gm129

Plasmid

#53765

Purpose

mammalian expression of EGFP-Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWPI-N3ICD

Plasmid

#185524

Purpose

Constitutive overexpression of human NOTCH3 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH3 intracellular domain (NOTCH3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGM

Plasmid

#71250

Purpose

luciferase reporter with human GM-CSF promoter -627 to + 28

Depositor

Peter Cockerill

Article

Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

Insert

GM-CSF promoter -627 to +28 fragment (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

GM-CSF

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pIALD2HMGr

Plasmid

#185867

Purpose

Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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eiABE

Plasmid

#227144

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS

Use

Tags

Expression

Mutation

D96R/E84R/V159R/H339A

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-pHmScarlet

Plasmid

#185538

Purpose

Expresses LGI1-pHmScarlet under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHmScarlet

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-pHluorin

Plasmid

#185537

Purpose

Expresses LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

Plasmid

#170808

Purpose

PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Inserts

GID1-VPR-IRES-HygR
GAI-tagBFP-Sa_dCas9 (GAI Synthetic, S. aureus, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Synonymous mutation in PYL1 to remove XhoI site (…

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-S473L-pHluorin

Plasmid

#185540

Purpose

Expresses mutant S473L LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed serine 473 for leucine

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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