We narrowed to 4,673 results for: pcr
-
Plasmid#36232DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-mCherry-ZeocinAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-2.5Kb MMP10
Plasmid#53973PurposeLuciferase reporter for mouse MMP10 2.5kb promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse MMP10 2.5Kb promoter (Mmp10 Mouse)
UseLuciferaseExpressionMammalianPromoterPCR from C57BL6/JAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB1 (human) HIS-tag pET
Plasmid#8520DepositorAvailable sinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)
Plasmid#44093DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, 12xHis tag, ADH1 transcription term…Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM342
Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.ExpressionBacterialAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-CXCR4(90)
Plasmid#98962PurposeMammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR4 (CXCR4 Human)
UseCRISPRExpressionMammalianMutationEncodes a gRNA for CRISPR-mediated targeting of C…PromoterU6Available sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
human PNP non-tagged
Plasmid#64199PurposeExpression of native human PNP (non-tagged)DepositorAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
L0-I35 p35S-XVE-pEstradiol GG0-GG1
Plasmid#195904PurposeLevel 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)DepositorInsertp35S-XVE-LexA-p35S
UseSynthetic BiologyAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
T-STOP sgRNA-1
Plasmid#83346PurposeThe sgRNA at the stop codon of human Brachyury(T) locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT-STOP sgRNA
ExpressionMammalianPromoterU6Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa1400-1648
Plasmid#19145DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
TagsMycExpressionMammalianMutationhuman BRG1 aa 1400-1648 (PCR product)Available sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
TOPO 2-5
Plasmid#68408PurposeTOPO backbone for GMAP genes with sites #2 and #5DepositorTypeEmpty backboneUseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)
Plasmid#44071DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)
Plasmid#44089DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#54472PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP(159-238)-gamma -2 (GNG2 Aequorea victoria, Human)
TagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-2 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTopo pLox hPGK-Puro pA pLox
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxExpressionMammalianAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…ExpressionBacterialAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ038
Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttRNA-gRNA scaffold
UseSynthetic BiologyAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARF4-Myc
Plasmid#67350PurposeMammalian expression of hArf4 with C-term Myc tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARF4 (ARF4 Human)
TagsMycExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU1
Plasmid#69316PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfpDepositorInsertGFPint-FgF
ExpressionBacterialAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only