165,567 results

1. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJL4

   Plasmid

   #199379

   Purpose

   Encodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   jGCaMP7f::SL2::mScarlet-I
   

   Expression

   Bacterial

   Mutation

   no mutations

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

   Plasmid

   #73796

   Purpose

   pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

   Depositor

   Ronald Davis

   Article

   Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

   Inserts

   dCas9-Mxi1
   Tet Repressor
   Structural gRNA for S pyogenes
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1, pRPR1(TetO), and pTef1

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Justin English, Fleur Ferguson

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRANS_250d

   Plasmid

   #91124

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   PvUbi2

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGKp-GFP-YAP (5SA)

   Plasmid

   #174174

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Promoter

   UbC

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. IGF2BP1

    Plasmid

    #155676

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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