162,408 results

1. ISceI-GR-RFP

   Plasmid

   #17654

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   I-SceI-GRLBD (SCEI, Nr3c1 Budding Yeast, Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   C655G mutation in the glucocorticoid receptor (se…

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV_VSBV_G

   Plasmid

   #207312

   Purpose

   Mammalian expression of Variegated Squirrel Bornavirus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSBV_G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HBV 1.3-mer X-null replicon

   Plasmid

   #65461

   Purpose

   containing 1.3 units of the X-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

   Insert

   1.3 units of the X-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

   Plasmid

   #141372

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. MSCV-Cre

   Plasmid

   #24064

   Purpose

   Retroviral coexpression of Cre and EGFP

   Depositor

   Dan Littman

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MESV 5' LTR

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFV2-HA

   Plasmid

   #187082

   Purpose

   Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

   Depositor

   Steven Vik

   Article

   Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

   Insert

   atpBEFHAGDC
   

   Use

   Tags

   6-His beta subunit (atpD) and HA tag subunit a (a…

   Expression

   Bacterial

   Mutation

   All Cys codons changed to Ala.

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-SF2

   Plasmid

   #17990

   Depositor

   Tom Misteli

   Article

   Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

   Insert

   SF2 (SRSF1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains SF2 (aa1-210)

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-mEB3-eGFP-6xHis

   Plasmid

   #130983

   Purpose

   Bacterial expression of mouse EB3-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB3-GFP-6xHis (Mapre3 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. STING-V1

   Plasmid

   #124262

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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