62,252 results

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pCEFL EGFP-TEADi

Plasmid

#140144

Purpose

TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001

Plasmid

#139311

Purpose

Recruitable PI4KA catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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p-dCas9-TET1-Hygro

Plasmid

#104404

Purpose

transient expression of dCas9-TET1 fusion protein

Depositor

Stephan Beck

Insert

TET1 (TET1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-clathrin

Plasmid

#136563

Purpose

Clathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-clathrin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-DeltaRBP

Plasmid

#135999

Purpose

G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C-term of G3BP1 Deleted for Delta RBP

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

Plasmid

#128386

Purpose

Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

Depositor

Craig Duvall

Article

Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

Inserts

NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

C-terminal luc2 fragment and N-terminal luc2 frag…

Expression

Mammalian

Mutation

N terminal carbohydrate recognition domain fused …

Promoter

EF1A and IRES

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

Plasmid

#134645

Purpose

lysosome-targeted Sac1 catalytic domain wild type

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

Sac1 catalytic domain (77-520 aa) (SACM1L Human)

Use

Lentiviral

Tags

FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

Expression

Mammalian

Mutation

contains only amino acids 77-520 (Please see depo…

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET-EIN-AlkBH5(66-292)

Plasmid

#125732

Purpose

Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

Depositor

Vincenzo Venditti

Article

Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

Insert

AlkBH5(66-292) (ALKBH5 Human)

Tags

6xHis and EIN

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_UCP1

Plasmid

#132274

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP1 (UCP1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pC0096 RESCUE truncation (Cas13 delta 892-1095)

Plasmid

#130663

Purpose

CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b(delta 892-1095)-ADAR2dd

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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