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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OTmut

Plasmid

#185388

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only
RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pXJ40-GFP-iBox-PAK4cat

Plasmid

#190179

Purpose

expresses GFP-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

GFP-iBox-PAK4cat

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-ccdB

Plasmid

#14154

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_67_pKD46.10

Plasmid

#178942

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pT2/SVNeo

Plasmid

#26553

Depositor

Perry Hackett

Article

Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

Insert

pT2/SVNeo

Expression

Bacterial

Mutation

added the Rmut(80, 87, d101)

Available Since

March 14, 2012

Availability

Academic Institutions and Nonprofits only

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