We narrowed to 8,358 results for: BLI;

1. pAG416GAL-3x-uC-HCoV-229E_3CL

   Plasmid

   #201937

   Purpose

   Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Insert

   HCoV-229E 3CL protease
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGMC00024 (aka pCW-Cas9n-Blast)

   Plasmid

   #195315

   Purpose

   Dox inducible lentiviral Cas9 nickase

   Depositor

   Raj Chari

   Insert

   Cas9-nickase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEXP1-DEST-Noa1-HIS

   Plasmid

   #191972

   Purpose

   Destination vector containing HIS-tagged NOA1 coding sequence.

   Depositor

   Luca Scorrano

   Insert

   Noa1-HIS (NOA1 Human)
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PARL-D319N-FLAG C-t

   Plasmid

   #191957

   Purpose

   Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant

   Depositor

   Luca Scorrano

   Insert

   PARL D319N (PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. R6K_tac_dGFPmut3_ChInt

   Plasmid

   #187388

   Purpose

   chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

   Depositor

   Roi Avraham

   Insert

   dGFPmut3_AAV
   

   Use

   Tags

   AAV

   Expression

   Bacterial

   Mutation

   C-terminal AAV tail

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. KBB96

   Plasmid

   #185113

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains

   Depositor

   Ken Belanger

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Last FXFG domain of Nup1

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KBB109

   Plasmid

   #185066

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site

   Depositor

   Ken Belanger

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal region, truncated at NsiI restric…

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZRS-TrkC-mCherry

   Plasmid

   #182949

   Purpose

   To express the rat TrkC fused in the C-terminus with mCherry

   Depositor

   Pantelis Tsoulfas

   Insert

   TRk-C-mCherry (Ntrk3 Rat, Synthetic)
   

   Use

   Retroviral and Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJA12 Mamu-A1*001:01

   Plasmid

   #180442

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   Mamu-A1*001:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid

    #182338

    Purpose

    Template plasmid for PCR amplification of initial recombineering cassette

    Depositor

    Mario Capecchi

    Insert

    BSD
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid

    #177208

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJA13 Mamu-A1*002:01

    Plasmid

    #180443

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-A1*002:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTCF122 Mamu-B*008:01

    Plasmid

    #180455

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-B*008:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTCF194 Mamu-E*02:04

    Plasmid

    #180461

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-E*02:04
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SYKA-c014

    Plasmid

    #175488

    Purpose

    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-Zbasic-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV V5-Senp2

    Plasmid

    #175118

    Purpose

    Lentiviral expression of V5-tagged mouse Senp2

    Depositor

    Larry Gerace

    Insert

    Senp2 (Senp2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RAB5AA-c006

    Plasmid

    #175476

    Purpose

    Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSNA-c007

    Plasmid

    #175467

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies.

    Depositor

    Opher Gileadi

    Insert

    MSN (MSN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid

    #178046

    Purpose

    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Renilla_713 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid

    #178045

    Purpose

    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Luciferase_1309 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKSB- attP 3xP3-GFPsv40 GGGG AAGA

    Plasmid

    #174534

    Purpose

    attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    attP and 3xP3-GFP-SV40 BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKSB- PUb-GFPsv40 GGGG AAGA

    Plasmid

    #174536

    Purpose

    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    PUb-GFP-SV40 BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid

    #174537

    Purpose

    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator

    Depositor

    Eric Marois

    Insert

    PUb-GFP-Tub56Dterminator BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pISV-DsRed1-nopP

    Plasmid

    #171025

    Purpose

    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.

    Depositor

    Christian Staehelin

    Insert

    nopP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2 X EN 35S

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pANY4-pL18-ccdB

    Plasmid

    #164898

    Purpose

    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #137857

    Purpose

    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. rox Neo* rox

    Plasmid

    #134322

    Purpose

    rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXZ13lacZalphaKanR

    Plasmid

    #160230

    Purpose

    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid

    #91803

    Purpose

    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX335 Mouse 5' Srcap gRNA B

    Plasmid

    #127905

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX335 Mouse 5' Srcap gRNA A

    Plasmid

    #127904

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pX330 Mouse 3' Ubf gRNA

    Plasmid

    #127902

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Mouse 3' Ubf
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSB700-TTN-Puro

    Plasmid

    #128323

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against human TTN for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CMV-Lin28a-as

    Plasmid

    #120528

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Fly 2.0 pIDTBlue

    Plasmid

    #117418

    Purpose

    Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

    Depositor

    Dirk Dittmer

    Insert

    FLY 2.0
    

    Use

    Tags

    NA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CAG-EGFP-Lin28IR

    Plasmid

    #120517

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. U6Lin28a-as

    Plasmid

    #120536

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. U6-Lin28a-s

    Plasmid

    #120535

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge