We narrowed to 4,324 results for: Pet2

1. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Jean-Baptiste Boulé, Virgina Zakian

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Tags

   histag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-HIS-Xyl-Xmod-DocIII

   Plasmid

   #60865

   Purpose

   Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   Xyl
   Xmod-DocIII
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Threonine 120 to Cysteine in Xylanase domain

   Promoter

   T7

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mTagBFP2-TEV-Strep-His

   Plasmid

   #210824

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mTagBFP2
   

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-Tev-Keap1

   Plasmid

   #62456

   Purpose

   His6-halo tagged human Keap1 protein for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Halo-Tev

   Expression

   Bacterial

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28 E2 LBD-Tev-E1a-His

   Plasmid

   #232117

   Purpose

   For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   DBT (DBT Human)
   

   Tags

   6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+) NRP1 b1b2 (273-586)

   Plasmid

   #162733

   Purpose

   Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1b2 domains (residues 273-586)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   March 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28a-GGG-ELP(120nm)-Cys

   Plasmid

   #91572

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(120nm)-Cys
   

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-41-4GS-LaG-2

   Plasmid

   #172766

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41-4GS-LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a His tag-FRB-Venus C

   Plasmid

   #100980

   Purpose

   Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

   Depositor

   Heinrich Leonhardt

   Article

   Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

   Insert

   His-FRB-Venus C
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204403

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid

    #101239

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175783

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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