Addgene

We narrowed to 13,554 results for: TIM;

Showing: 1761 - 1780 of 13554 results

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pZE-PpadC-yveF-yveG-egfp

Plasmid

#201791

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PpadC-yveF-yveG

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Use

Tags

3xFlag, His, and T7

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise

Plasmid

#103972

Purpose

Plasmid encoding mTurquoise

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3 (3A)

Plasmid

#14964

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (3A) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Three Ser to Ala mutations in the phosphorylation…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Use

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-C1

Plasmid

#60558

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pVHed05

Plasmid

#82351

Purpose

Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

TP901-1 integrase
Bxb1 integrase
AraC (araC )
TetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-OX

Plasmid

#184615

Purpose

For expression of a fluorescent sensor for orexin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDA183

Plasmid

#74247

Purpose

pSTL1-dPSTRr(2,1)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRr(2,1)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 pS43 METTL3

Plasmid

#200732

Purpose

Bacterial expression of GST-Tagged codon optimized human pS43 METTL3

Depositor

Claudio Alarcón

Insert

pS43 METTL3 (METTL3 Human)

Use

Tags

GST from backbone plasmid

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli, UAG co…

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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