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plYJP067-Pttg

Plasmid

#202300

Purpose

PTtg-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTtg-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT238-Pbad

Plasmid

#202298

Purpose

PBad-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pbad-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT237-Ptac

Plasmid

#202297

Purpose

PTac-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTac-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT475

Plasmid

#202292

Purpose

RPU plasmid w/ high RBS (p15a backbone)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN BFP

Plasmid

#188644

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

BFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-AM

Plasmid

#153427

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-LO

Plasmid

#153428

Purpose

Retroviral (MSCV) expression of LSSmOrange fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

LSSmOrange

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MtMscL delta49

Plasmid

#118342

Purpose

M. tuberculosis MscL (delta49) in pET19b

Depositor

Douglas Rees

Article

Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

Insert

MscL (mscL Mycobacterium tuberculosis)

Tags

6x His Tag and Thrombin Cleavage site

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 102 to Stop Codon

Promoter

T7 Promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHYBAD94

Plasmid

#42305

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEC6

Tags

GB1-H6

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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human pirh2-(138-261)

Plasmid

#24882

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 138-261.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
human pirh2-(187-261)

Plasmid

#24883

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 187-261.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-BRX-eGFP-NLS

Plasmid

#217534

Purpose

AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

BMP reporter element and miniXon casette (Smad1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

BMP Reporter Element

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0039 DisCas7-11 only expression with CRISPR array

Plasmid

#172500

Purpose

Encodes the DisCas7-11 protein only for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 only expression with CRISPR array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172503

Purpose

Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE PGK-GFP-IRES-LUC-W

Plasmid

#46793

Purpose

2nd generation lentiviral vector

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Inserts

PGK
eGFP (eGFP )
IRES
Luciferase

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2

Plasmid

#244104

Purpose

Bacterial expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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FH-TET3-pEF

Plasmid

#49446

Purpose

This plasmid expresses full-length human TET3.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

Tet methylcytosine dioxygenase 3 (TET3 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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