159,333 results

1. pDEST17-UbE2A

   Plasmid

   #15780

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2A (UBE2A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-synapsin-HaloCaMP1a-EGFP

   Plasmid

   #138327

   Purpose

   chemigenetic calcium indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Has Service

   AAV1 and AAV9

   Insert

   HaloCaMP1a
   

   Use

   AAV

   Tags

   EGFP, His-tag, and Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Frb-NLS-mCherry

   Plasmid

   #217613

   Purpose

   part B of SPARK-OFF

   Depositor

   Xiaokun Shu

   Article

   Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

   Insert

   Frb-NLS-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SIRT6 Flag

   Plasmid

   #13817

   Purpose

   Mammalian expression of human SIRT6 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a-hPKM2

   Plasmid

   #44242

   Depositor

   Matthew Vander Heiden, Lewis Cantley

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

   Insert

   Pyruvate Kinase M2 (PKM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU44

   Plasmid

   #167666

   Purpose

   ENTRY vector for gRNA4 cloning under pAtU3 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Expression

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-LoxP-PURO-LoxP-SNAP-TERT

   Plasmid

   #71390

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK02

    Plasmid

    #105133

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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