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Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
UseTagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, ā¦PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-nrip1b
Plasmid#192732PurposeGateway entry vector encoding zebrafish nrip1bDepositorInsertnrip1b (nrip1b Zebrafish)
UseGateway entry vectorTagsExpressionMutationL661P (rs511527097); S720P; A801T; K862E; A863P; ā¦PromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-Vector-Dendra2
Plasmid#97101Purposeempty vector for overexpression of microRNA and Dendra2 in ZebrafishDepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHD066
Plasmid#84032PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and ElastaseAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR - CaSR-EGFP
Plasmid#191503PurposeA zebrafish CaSR-EGFP construct used for Gateway cloning.DepositorInsertCaSR-EGFP-SV40
UseZebrafish expressionTagsExpressionMutationPromoternoneAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3āend of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-eab2-[GTR]
Plasmid#46144DepositorInsertEGFP/mCherry
UseCre/Lox; Tol2 zebrafish transgenic expressionTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stableā¦TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-eab2-GG
Plasmid#182154PurposeExpression of artificial intron separated eGFPDepositorInsertGG
UseZebrafish expressionTagsExpressionMutationPromotereab2Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-eab2-AA
Plasmid#182153PurposeExpression of artificial intron separated mAppleDepositorInsertAA
UseZebrafish expressionTagsExpressionMutationPromotereab2Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+methylated-reporter
Plasmid#164491PurposeReporter to test methylated mRNA stability, for mRNA injectionDepositorInsertA-less CDS, HA tag, 12x m6A motif 3'UTR
UseProduction of mrna for zebrafish injection.TagsHA tagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-ythdf2-3xflag
Plasmid#164489PurposeExpresses zebrafish ythdf2 with 3x flag tag, for mRNA injectionDepositorInsertythdf2 (ythdf2 Zebrafish)
UseProduction of mrna for zebrafish injection.Tags3x-flagtagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry
Plasmid#230062PurposeExpression of genetically encoded calcium chelator in zebrafish hepatocytes.DepositorInsertSpiCee
UseZebrafish expressionTagsFLAG and mRFP1ExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-OXER1-mKate2
Plasmid#226713Purposeoverexpression red fluorescent OXER1 protein in zebrafish larvaeDepositorInsertOXER1 (OXER1 Human)
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-ythdf3-3xflag
Plasmid#164490PurposeExpresses zebrafish ythdf3 with 3x flag tag, for mRNA injectionDepositorInsertythdf3 (ythdf3 Zebrafish)
UseProduction of mrna for zebrafish injection.Tags3x-flagtagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax
Plasmid#222138PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 (cas9 Synthetic)
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only