We narrowed to 1,094 results for: gibson assembly
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Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R265
Plasmid#138167PurposeExpresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mTagBFP2
Plasmid#68413PurposeTOPO construct expressing mTagBFP2 for generation of GMAP-compatible geneDepositorInsertmTagBFP2
UseGmapTags6xHisExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO ZeoR
Plasmid#68446PurposeTOPO construct expressing ZeoR for generation of GMAP-compatible geneDepositorInsertZeocin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO HygroR
Plasmid#68445PurposeTOPO construct expressing HygroR for generation of GMAP-compatible geneDepositorInsertHygromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast p5
Plasmid#68470PurposeInverted and floxed blasticidin resistanceDepositorInsertBlasticidin Resistance
UseCre/LoxExpressionMammalianMutationInvertedPromoterLTRAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO iRFP670
Plasmid#68443PurposeTOPO construct expressing iRFP670 for generation of GMAP-compatible geneDepositorInsertiRFP670
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKO2
Plasmid#68442PurposeTOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible geneDepositorInsertmKusabira-Orange2
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO Venus
Plasmid#68493PurposeTOPO construct expressing Venus for generation of GMAP-compatible geneDepositorInsertsplit Venus
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
EZ-L498
Plasmid#131771PurposePPGK1_FUSN_Citrine _PixE_TCYC1DepositorInsertPPGK1_FUSN_Citrine _PixE_TCYC1
UseIntegration into his3 locusExpressionYeastPromoterPGKAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
TagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CloverCP
Plasmid#68444PurposeTOPO construct expressing CloverCP for generation of GMAP-compatible geneDepositorInsertCloverCP
UseGmapTags"CP" degradation domainExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO mT2
Plasmid#68491PurposeTOPO construct expressing mT2 for generation of GMAP-compatible geneDepositorInsertmTurquoise2
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-PIPKγ87
Plasmid#242824PurposeEncodes wildtype human PIPKγ87 and N-terminal EBFP2 tagDepositorInsertPhosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
ExpressionMammalianMutationN/AAvailable SinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSuper TR G414C
Plasmid#207612PurposeU3 promoter driven expression of the telomerase RNA with a G414C CAB box mutationDepositorInsertTelomerase RNA G414C
ExpressionMammalianPromoterU1Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Lg
Plasmid#216977PurposeExpression of GvpT-Linker-Large BiTDepositorInsertGvpT-Linker-Large BiT
TagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaRLS
Plasmid#216955PurposeMega' with knockout of GvpR, GvpL and GvpSDepositorInsertMega' with knockout of GvpR, GvpL and GvpS
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBSU
Plasmid#216953PurposeMega' with knockout of GvpB, GvpS and GvpUDepositorInsertMega' with knockout of GvpB, GvpS and GvpU
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaLSK
Plasmid#216957PurposeMega' with knockout of GvpL, GvpS and GvpKDepositorInsertMega' with knockout of GvpL, GvpS and GvpK
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBRG
Plasmid#216945PurposeMega' with knockout of GvpB, GvpR and GvpGDepositorInsertMega' with knockout of GvpB, GvpR and GvpG
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-U
Plasmid#217003PurposeExpression of Small BiT-Linker-GvpUDepositorInsertSmall BiT-Linker-GvpU
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBRS
Plasmid#216947PurposeMega' with knockout of GvpB, GvpR and GvpSDepositorInsertMega' with knockout of GvpB, GvpR and GvpS
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBS
Plasmid#216895PurposeMega' with knockout of GvpB and GvpSDepositorInsertMega' with knockout of GvpB and GvpS
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBLJ
Plasmid#216951PurposeMega' with knockout of GvpB, GvpL and GvpJDepositorInsertMega' with knockout of GvpB, GvpL and GvpJ
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBU
Plasmid#216899PurposeMega' with knockout of GvpB and GvpUDepositorInsertMega' with knockout of GvpB and GvpU
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBJ
Plasmid#216897PurposeMega' with knockout of GvpB and GvpJDepositorInsertMega' with knockout of GvpB and GvpJ
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBL
Plasmid#216894PurposeMega' with knockout of GvpB and GvpLDepositorInsertMega' with knockout of GvpB and GvpL
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBG
Plasmid#216893PurposeMega' with knockout of GvpB and GvpGDepositorInsertMega' with knockout of GvpB and GvpG
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-G-Sm
Plasmid#216990PurposeExpression of GvpG-Linker-Small BiTDepositorInsertGvpG-Linker-Small BiT
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBT
Plasmid#216898PurposeMega' with knockout of GvpB and GvpTDepositorInsertMega' with knockout of GvpB and GvpT
ExpressionBacterialAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBN
Plasmid#216891PurposeMega' with knockout of GvpB and GvpNDepositorInsertMega' with knockout of GvpB and GvpN
ExpressionBacterialAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBR
Plasmid#216890PurposeMega' with knockout of GvpB and GvpRDepositorInsertMega' with knockout of GvpB and GvpR
ExpressionBacterialAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-N-Lg
Plasmid#216963PurposeExpression of GvpN-Linker-Large BiTDepositorInsertGvpN-Linker-Large BiT
TagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Sm
Plasmid#217000PurposeExpression of GvpT-Linker-Small BiTDepositorInsertGvpT-Linker-Small BiT
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBF
Plasmid#216892PurposeMega' with knockout of GvpB and GvpFDepositorInsertMega' with knockout of GvpB and GvpF
ExpressionBacterialAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-J
Plasmid#216999PurposeExpression of Small BiT-Linker-GvpJDepositorInsertSmall BiT-Linker-GvpJ
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only