We narrowed to 9,217 results for: Coli

1. pJ211_rha_WT_low

   Plasmid

   #128851

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ211_rha_WT_med

   Plasmid

   #128852

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Strep-TGT-His

   Plasmid

   #206495

   Purpose

   Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

   Insert

   E. coli tRNA Guanine Transglycosylase
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ211_WT_med

   Plasmid

   #128849

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJ211_rha_WT_hi

   Plasmid

   #128853

   Purpose

   High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SSB-wt

   Plasmid

   #197260

   Purpose

   wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

   Depositor

   Timothy Lohman

   Article

   Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

   Insert

   wt E. coli SSB
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pACM4-ACA

   Plasmid

   #49809

   Purpose

   Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   ACP transacylase
   Acetyl-CoA carboxylase
   acetyl-CoA carboxylase
   Acetyl-CoA carboxylase
   Acetyl-CoA carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Silent site-directed mutagenesis to remove SalI s…

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCK314

   Plasmid

   #110545

   Purpose

   Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   Modified PrhaBAD (CRP-binding sites removed)
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMQ430

    Plasmid

    #42216

    Depositor

    Martin Marinus

    Article

    Calmann et al J Bacteriol. 2003 Aug;185(16):5012-4.

    Insert

    dam (dam E. coli K-12)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. His8:MBP-tev-Gly-eK-Flv

    Plasmid

    #83383

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His8:MBP-tev-Trp-eK-Flv

    Plasmid

    #83390

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His8:MBP-tev-Asp-eK-Flv

    Plasmid

    #83389

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. His8:MBP-tev-His-eK-Flv

    Plasmid

    #83388

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His8:MBP-tev-Lys-eK-Flv

    Plasmid

    #83387

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His8:MBP-tev-Leu-eK-Flv

    Plasmid

    #83386

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His8:MBP-tev-Arg-eK-Flv

    Plasmid

    #83385

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His8:MBP-tev-Phe-eK-Flv

    Plasmid

    #83384

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JM3 [JBEI-6891]

    Plasmid

    #89788

    Purpose

    Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

    Depositor

    Harry Beller

    Article

    Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

    Insert

    tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge