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Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
UseTagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
UseTagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBD
Plasmid#232985PurposeGalactose iduced expression of Gcn4 DBD in yeastDepositorInsertGcn4 DBD
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124I
Plasmid#232964PurposeGalactose iduced expression of Gcn4 F124I in yeastDepositorInsertGcn4 F124I
UseTagsTEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3HA
Plasmid#232998PurposeGalactose iduced expression of Gcn4 k0.47HA in yeastDepositorInsertGcn4 k0.47
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDGFP
Plasmid#232999PurposeGalactose iduced expression of Gcn4 DBDGFP in yeastDepositorInsertDBD
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WT 30mer
Plasmid#231888PurposeBacterial expression of N-terminally 6His tagged Gcn4 WT 30merDepositorInsertGcn4 30mer
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_DBD
Plasmid#231886PurposeBacterial expression of N-terminally 6His tagged Gcn4 DBDDepositorInsertGcn4 DBD
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Swap1
Plasmid#231879PurposeBacterial expression of N-terminally 6His tagged Gcn4 Swap1DepositorInsertGcn4 Swap1
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 R3
Plasmid#231874PurposeBacterial expression of N-terminally 6His tagged Gcn4 R3DepositorInsertGcn4 R3
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationD103R, D133R, K143RPromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 E3
Plasmid#231863PurposeBacterial expression of N-terminally 6His tagged Gcn4 E3DepositorInsertGcn4 E3
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationD105E, D118E, D139EPromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 W120A
Plasmid#231862PurposeBacterial expression of N-terminally 6His tagged Gcn4 W120ADepositorInsertGcn4
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationW120APromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Med15
Plasmid#231887PurposeBacterial expression of N-terminally 6His tagged Gcn4 Med15DepositorInsertMed15
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 F108A+
Plasmid#231875PurposeBacterial expression of N-terminally 6His tagged Gcn4 F108A+DepositorInsertGcn4 F108A+
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationF108A, T121L, A141PPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 R2
Plasmid#231870PurposeBacterial expression of N-terminally 6His tagged Gcn4 R2DepositorInsertGcn4 R2
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationK118R, K140RPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 L123A+
Plasmid#231869PurposeBacterial expression of N-terminally 6His tagged Gcn4 L123A+DepositorInsertGcn4 L123A+
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationL123APromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only