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Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK297
Plasmid#59566PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-sfGFP-24xGCN4_v1
Plasmid#61056PurposeA direct fusion of sfGFP to the 24xGCN4_v1 peptide arrayDepositorInsertsfGFP-24xGCN4_v1 peptide array
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_pBabePuro
Plasmid#58422Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids P29 to D408PromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB556
Plasmid#115966PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/15 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Mouse)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-MEF2D
Plasmid#32964PurposeFor mammalian expression of mouse MEF2D with a 3x FLAG tagDepositorInsertMEF2D (Mef2d Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro
Plasmid#58492Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationdeleted amino acids P29 to D408 and bearing mutat…PromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-GFP
Plasmid#89344Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFPDepositorUseLentiviralTagsSNAPf tag and mEGFPExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)
Plasmid#78509PurposeConstruct for expressing CD45R0 (extracellular domain) His10 in Sf9DepositorInsertCD45R0Ex (PTPRC Human)
UseTagsHis10 and SNAPfExpressionInsectMutationPromoterAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PERK 4: PERKKD-pGEX4T-1
Plasmid#21817DepositorInsertPERK (537-1114) (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114.PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-3p
Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK299
Plasmid#59568PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4DepositorInsertSGP(14) Kozak L7Ae-P2A-EYFP 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 S383A
Plasmid#27160PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationTAD mutant: S383APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB332
Plasmid#115949PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR-Gly9-DDX6 (contains SapI)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW122
Plasmid#115962PurposeORF insert for replicon MoCloDepositorInsertLvl0 5'-2xTetApt-mVenus-PEST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S_C56S-pFLAG-CMV1
Plasmid#31383DepositorInsertPDI1A (P4HB Human)
UseTagsFLAG M2ExpressionMammalianMutationC-terminal cysteines of both active sites mutated…PromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only