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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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zebrafish SLC2A2 (zfSLC2A2)

Plasmid

#65103

Purpose

Full length SLC2A2 cDNA (Danio rerio)

Depositor

Josep Planas

Publication

Castillo et al Am J Physiol Regul Integr Comp Physiol.

Insert

SLC2A2 (slc2a2 Zebrafish)

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
Rab43 cds in pGem-T Easy

Plasmid

#80565

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab43 (rab43 Zebrafish)

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Rab20 cds in pGem-T Easy

Plasmid

#80540

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab20 (rab20 Zebrafish)

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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miR-223-RFP pCDNA

Plasmid

#97142

Purpose

expression of miR-223 and TagRFP

Depositor

Qing Deng

Publication

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Tags

TagRFP

Expression

Mammalian

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Cul1b-3'UTR

Plasmid

#97153

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Publication

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Cul1b 3'UTR

Expression

Mammalian

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Traf6-3'UTR

Plasmid

#97154

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Publication

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Traf6 3'UTR

Expression

Mammalian

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod2 guide

Plasmid

#107604

Purpose

An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod2 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod1 guide

Plasmid

#107603

Purpose

An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-uqcrc1 guide

Plasmid

#107602

Purpose

An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

uqcrc1 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) C49S

Plasmid

#110071

Purpose

Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Publication

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Tags

His6

Expression

Bacterial

Mutation

Cysteine 49 to Serine

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSi-check2-zRac2 - mut 3'UTR

Plasmid

#97159

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Publication

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dre-Rac2 3'UTR

Expression

Mammalian

Mutation

miR-722 Binding Mutant

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 5kb promoter/AcGFP

Plasmid

#98101

Purpose

Zebrafish aBb crystallin 5kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 5 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 4kb promoter/AcGFP

Plasmid

#98100

Purpose

Zebrafish aBb crystallin 4kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 4 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSi-check2-zRac2 3'UTR

Plasmid

#97158

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Publication

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dre-Rac2 3'UTR

Expression

Mammalian

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;mafbbP1Egfp

Plasmid

#90163

Purpose

contains mafbb specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbP1Egfp

Tags

EGFP

Expression

Bacterial

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5CE1-basEgfp

Plasmid

#90138

Purpose

contains snx5 enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5E1basEgfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;cldn5bCE1-basEgfp

Plasmid

#90136

Purpose

contains cldn5b enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1basEgfp (cldn5b Zebrafish)

Tags

EGFP

Expression

Bacterial

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMEsnx5P1gfp

Plasmid

#90131

Purpose

Gateway Mid-entry clone containing promoter sequence specific for snx5 driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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