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We narrowed to 3,980 results for: Bre;

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37C-A44C

Plasmid

#162582

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Alanine 37 changed to Cysteine and Alanine 44 cha…

Promoter

polyhedring

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_Q141V-P221L

Plasmid

#162584

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Glutamine 141 changed to Valine and Proline 221 c…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_L144C-P221C

Plasmid

#162585

Purpose

Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Leucine 144 changed to Cysteine and Proline 221 c…

Promoter

polyhderin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_G131C-N172C

Plasmid

#162586

Purpose

Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Glycine 131 changed to Cysteine and Asparagine 17…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N167C-L169C

Plasmid

#162587

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Asparagine 167 changed to Cysteine and Leucine 16…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Mad3 [N129A/6R]

Plasmid

#140075

Purpose

Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 S3070F

Plasmid

#139325

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 S3070F using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 S1363L

Plasmid

#139329

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 S1363L using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E1754K

Plasmid

#139330

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E1754K using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA FANCD2 Q223Stop

Plasmid

#139332

Purpose

Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert FANCD2 Q223Stop using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80D

Plasmid

#129516

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Aspartic acid

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1-Y80F

Plasmid

#129517

Purpose

Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 80 to Phenylalanine

Promoter

cmv

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/7R]

Plasmid

#114482

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5 (R511C)-V5 blast

Plasmid

#83104

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Arginine 511 to Cysteine

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5-V5 blast

Plasmid

#83100

Purpose

Lentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (T288P)-V5 blast

Plasmid

#83101

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (A270T)-V5 blast

Plasmid

#83102

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Alanine 270 to Threonine

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3

Plasmid

#112843

Purpose

transient/stable overexpression of the C-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C2 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

Plasmid

#112841

Purpose

transient/stable overexpression of the N-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-Puro

Plasmid

#110849

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-Puro

Plasmid

#110850

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-PGK-Puro

Plasmid

#110855

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-VRER-PGK-Puro

Plasmid

#110856

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Promoter

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge