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We narrowed to 5,049 results for: golden gate

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Showing: 4451 - 4500 of 5049 results

  6. pCHW00010

    Plasmid
    #222435
    Purpose
    Level 0, promoter module (GGAG-AATG) containing CBS4 and tomato miniDFR promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi
    Insert
    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    222435-plasmid-map-sequence-id-472307

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  12. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Expression
    Mammalian
    Promoter
    None
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

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  27. pJB50

    Plasmid
    #226311
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226311-plasmid-map-sequence-id-449923

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  28. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

    Plasmid
    #221994
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221994-plasmid-map-sequence-id-438723

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  29. TL1 alphaDdCBE 1-2 (right, DddA11-N)

    Plasmid
    #222000
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222000-plasmid-map-sequence-id-438640

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  30. pTE5418_pMBP-GlcR

    Plasmid
    #221192
    Purpose
    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    glcR (pden4400 from Paracoccus denitrificans)
    Tags
    10x His tag with E. coli maltose binding protein …
    Expression
    Bacterial
    Promoter
    T7 promoter and T7 promoter-lacO
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221192-plasmid-map-sequence-id-446698

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  31. pTE5419_p3WJdB-glcO36

    Plasmid
    #221193
    Purpose
    GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO36
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter with downstream glcO36 operator
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221193-plasmid-map-sequence-id-441880

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  35. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Expression
    Yeast
    Promoter
    GAL7
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219503-plasmid-map-sequence-id-435230

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  48. PAC_E83_LOV_eGFP

    Plasmid
    #231574
    Purpose
    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light
    Depositor
    Dominik Niopek
    Insert
    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    Expression
    Mammalian
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231574-plasmid-map-sequence-id-460758

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Showing: 4451 - 4500 of 5049 results