We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1-nAChr-Alpha6-CFP

   Plasmid

   #50482

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   CFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the CFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(-)-chicken myc-GPIX

   Plasmid

   #40913

   Depositor

   Renhao Li

   Insert

   chicken myc-GPIX (GP9 Chicken)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(-)-chicken Flag-GPIba

   Plasmid

   #40911

   Depositor

   Renhao Li

   Insert

   chicken Flag-GPIba
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

   Plasmid

   #194171

   Purpose

   Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-Associated Protein Tau 0N4R
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

   Plasmid

   #194680

   Purpose

   CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Hpep1-betaArrestin2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-CFP;UBC-Cre25nt

   Plasmid

   #65727

   Purpose

   Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Inserts

   CMV promoter driving CFP expression
   UBC promoter driving Cre-25nt expression
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and UBC

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225241

   Purpose

   Mammalian expression of iPASnFR-H in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-pcDNA3.1 Puro-CAG-Positron2

    Plasmid

    #239079

    Purpose

    Mammalian expression of positive-going voltage sensor

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    CAG

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225239

    Purpose

    Mammalian expression of iPASnFR-H in mitochondria

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225240

    Purpose

    Mammalian expression of iPASnFR-L in mitochondria

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

    Plasmid

    #182651

    Purpose

    human CD81-TEV cleavage site-FKBP-SmBiT tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225245

    Purpose

    Mammalian expression of iPASnFR-H in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-MUC2 D1 with cleavable His tag

    Plasmid

    #181813

    Purpose

    Expresses human MUC2 D1 with cleavable His tag, residues 21-389

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc2 D1 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 (+) -myc-OGA(GS-544)C181

    Plasmid

    #193996

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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