162,519 results

1. pHAGE-CDKN2A

   Plasmid

   #116726

   Purpose

   Lentiviral expression of CDKN2A

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDKN2A (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KHBD00727

   Plasmid

   #39748

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG9932 (CG9932 Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-copGFP

   Plasmid

   #73030

   Purpose

   Express copGFP

   Depositor

   Kazuhiro Oka

   Insert

   copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_TUBA1B sgRNA / hSpCas9

   Plasmid

   #172834

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSFFV_sfCherry3V(1-10)

   Plasmid

   #117483

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3V(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3V(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   6 substitutions (K45R, G52D, L83W, T106A, K182R, …

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tad-CBE(CCC5)

   Plasmid

   #235073

   Purpose

   Expresses Tad-CBE(CCC5) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCC5)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tad-CBE(TCA3)

   Plasmid

   #235064

   Purpose

   Expresses Tad-CBE(TCA3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCA3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tad-CBE(TCT1)

    Plasmid

    #235065

    Purpose

    Expresses Tad-CBE(TCT1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCT1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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