We narrowed to 115,040 results for: CIL

1. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

   Plasmid

   #123615

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A/K34A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A/K34A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-Soma-jGCaMP8f

   Plasmid

   #182943

   Purpose

   Cell body-targeted jGCaMP8f, under synapsin promoter.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   Soma-jGCaMP8f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mNeon-BlastR [M1G]

   Plasmid

   #171805

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-GUCY2C

   Plasmid

   #116749

   Purpose

   Lentiviral expression of GUCY2C

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   GUCY2C (GUCY2C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-mScarlet-BlastR [M1G]

   Plasmid

   #171814

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. px335 Mettl14 sgRNA #2

   Plasmid

   #61514

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gccgctcccggatctcctgc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. FUW TetO HA-AMPKa2 WT

   Plasmid

   #69826

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-IDR1-mCherry-Cry2

    Plasmid

    #177125

    Purpose

    Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (IDR1 domain) (YTHDC1 Human)
    

    Use

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    IDR1 domain only

    Promoter

    SFFV

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW TetO HA-AMPKa2 AS

    Plasmid

    #69827

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. px335 Mettl14 sgRNA #1

    Plasmid

    #61515

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gcggcagctcctagctcagc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. nCas9-UGI control for Target-AID (pRZ1047)

    Plasmid

    #131299

    Purpose

    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEAHISMRSA2028

    Plasmid

    #97001

    Purpose

    Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetro-CAG-DsRedExpress-G-TVA

    Plasmid

    #235698

    Purpose

    Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

    Depositor

    Hongjun Song, Guo-li Ming

    Article

    Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

    Insert

    DsRedExpress, TVA800, and Glyco
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-CIB1-mRuby-Rab35

    Plasmid

    #194334

    Purpose

    For use in light-induced Rab35 protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab35 (RAB35 Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDR111_mannose_Klf4Gata-3

    Plasmid

    #188401

    Purpose

    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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