We narrowed to 11,210 results for: nar

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pmiRFP670-PCNAL2 (pc3385)

Plasmid

#213140

Purpose

Expresses human PCNA miRFP670 tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2021 Dec 16;49(22):12

Insert

PCNA (PCNA Human)

Tags

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-RNAgateDel-sgEIF3D

Plasmid

#222137

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

V291-F306 deletion

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528D-S529D-sgEIF3D

Plasmid

#222127

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528D, S529D

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2386

Plasmid

#225639

Purpose

AAV plasmid with Ple388 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

AAV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a11helixDel-sgEIF3D

Plasmid

#222135

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

T317E, N320E, H321A

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-CtermDel-sgEIF3D

Plasmid

#222125

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

F527 truncation

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a5helixDel-sgEIF3D

Plasmid

#222133

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

D249Q, V262I, Y263A

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-FCPF

Plasmid

#220131

Purpose

Expresses dCas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SOX2_HDR

Plasmid

#163752

Purpose

gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SOX2 (SOX2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEndo-I-OnuI-TSM

Plasmid

#207954

Purpose

Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Insert

Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.

Use

Synthetic Biology

Expression

Bacterial

Mutation

Leucine 212 changed to proline, leucine 434 in fu…

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR101

Plasmid

#185046

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

Amino acids 2-8 deleted of L31p coding region

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR111

Plasmid

#185047

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

Last 8 amino acids deleted of L31p coding region

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR120

Plasmid

#185041

Purpose

J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-DCP1

Plasmid

#215843

Purpose

Expression of FLAG/HA-DCP1

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human DCP1 (DCP1B Human)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC7

Plasmid

#205060

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 7 aa

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC13

Plasmid

#205063

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 13 aa

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC17

Plasmid

#205061

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 17 aa

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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