Addgene

We narrowed to 11,638 results for: pcdna

Showing: 2701 - 2730 of 11638 results

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pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3 Flag MKK7b1

Plasmid

#14622

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1his p66shc S36A

Plasmid

#10973

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF37-2xCSTREP

Plasmid

#136198

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF37

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Rat NCX1.7 (pcDNA3.1+)

Plasmid

#75192

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7d

Plasmid

#51379

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7d (MIRLET7D Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-FLAG

Plasmid

#97071

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag PA1

Plasmid

#15559

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-TOP luciferase

Plasmid

#26611

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

eEF2 5'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-C-3XFLAG-h-Lmbr1l

Plasmid

#123462

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Limb development membrane protein 1 like (LMBR1L Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RecQL4-mVen

Plasmid

#211403

Purpose

mammalian expression of human RecQL4 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-cDAXX-0SB

Plasmid

#169728

Purpose

Mammalian expression construct of GFP tagged cDAXX-0SB

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX-0SB (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Comprises residues 495-741 and has the major SB m…

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-R482W

Plasmid

#32709

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R482W

Promoter

CMV

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Rs4084-RBD-8h

Plasmid

#161825

Purpose

Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Use

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T321-D519)

Promoter

CMV

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Rs4231-RBD-8h

Plasmid

#161824

Purpose

Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Use

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T320-D518)

Promoter

CMV

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3 1-448

Plasmid

#44033

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Use

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 1-448; V274D and S393G

Promoter

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dLZ

Plasmid

#176048

Purpose

Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Use

Tags

Expression

Mammalian

Mutation

deleted amino acids 414-433

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Synthetic, Human)

Use

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF63-2xCSTREP

Plasmid

#136223

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF63

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge