We narrowed to 11,954 results for: pcDNA

1. pcDNA5-FRT-TO-Ubc13

   Plasmid

   #212806

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT-TO-Mms2

   Plasmid

   #212807

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Mms2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.3-hALDOA K230A

   Plasmid

   #210811

   Purpose

   To block the formation of the initial Schiff base with FBP

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 230 of ALDOA mutated to alanine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA TfR1-PuRRRE

   Plasmid

   #208327

   Purpose

   C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TfR1-PuRRRE
   

   Use

   Tags

   PuRRRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA TfR1-TREER

   Plasmid

   #208325

   Purpose

   C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TfR1-TREER
   

   Use

   Tags

   TREER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA TfR1-TinyERRE

   Plasmid

   #208324

   Purpose

   C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TfR1-TinyERRE
   

   Use

   Tags

   TinyERRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TO-EGFP-N

   Plasmid

   #210270

   Purpose

   N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cacna2d1 BBS pcDNA3

   Plasmid

   #206106

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

   Depositor

   Annette Dolphin

   Article

   Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid

    #207152

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)G

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA TfR1-PunyEEER

    Plasmid

    #208326

    Purpose

    C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TfR1-PunyEEER
    

    Use

    Tags

    PunyEEER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3_ATP10B-G671R-N865K

    Plasmid

    #203699

    Purpose

    expresses ATP10B with the pathogenic mutations G671R and N865K

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G671R, N865K

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cav2.2 W391A pcDNA3

    Plasmid

    #206085

    Purpose

    expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

    Depositor

    Annette Dolphin

    Article

    Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-hARFRP1(T31N)-HA

    Plasmid

    #206401

    Purpose

    Mammalian expression of hARFRP1(T31N)

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T31N

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-hARFRP1(Q79L)-HA

    Plasmid

    #206400

    Purpose

    Mammalian expression of hARFRP1(Q79L)

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1+FLAG-KrasnatQ61R

    Plasmid

    #206843

    Purpose

    To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glutamine 61 to Arginine

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 NKX6-2-Venus

    Plasmid

    #203712

    Purpose

    Constitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 NKX6-2(1-40)-Venus

    Plasmid

    #203713

    Purpose

    SPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (1-40) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    deletion of amino acids 41-277

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Rat MMP-9 pcDNA3.1

    Plasmid

    #196951

    Purpose

    Plasmid expressing an antigen targeting the MMP9 metalloproteinase

    Depositor

    James Trimmer

    Insert

    mmp-9 (Mmp9 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cav2.2 HA W391A pcDNA3

    Plasmid

    #206071

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

    Depositor

    Annette Dolphin

    Article

    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP Cav2.2 HA pcDNA3

    Plasmid

    #206053

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. cacna2d1 HA D491A pcDNA3

    Plasmid

    #206070

    Purpose

    expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D491A

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA 3.1 - circMAN2A1

    Plasmid

    #198837

    Purpose

    This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

    Depositor

    Stefan Stamm

    Article

    Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

    Insert

    circular mannosidase alpha class 2A member 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circular RNA composed by 2 exons

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-INSRR-V5/HIS

    Plasmid

    #201981

    Purpose

    expression of human INSRR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSRR receptor tyrosine kinase (INSRR Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA5/FRT-ChemoB

    Plasmid

    #193808

    Purpose

    CMV overexpression of ChemoB in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA5/FRT-ChemoY

    Plasmid

    #193810

    Purpose

    CMV overexpression of ChemoY in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoY
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    Venus:A206K, T225R; HT7: E143R, E147R, L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA5/FRT-ChemoR

    Plasmid

    #193811

    Purpose

    CMV overexpression of ChemoR in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoR
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    mScarlet: D201K

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA 3.1-nsp7

    Plasmid

    #201026

    Purpose

    Expresses SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(-)-Green Falcan1

    Plasmid

    #199705

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet-ON

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3-AUP1 A397D

    Plasmid

    #185342

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    A397D

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3-hDHX58-VenusFlag

    Plasmid

    #197624

    Purpose

    Human LGP2 protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    DHX58 (DHX58 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Sur1-Flag/pCDNA3.1

    Plasmid

    #197294

    Purpose

    Plasmid expressing an antigen targeting Sur1

    Depositor

    William Coetzee

    Insert

    Sur1 (Abcc9 C. griseus (hamster))
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.4_1G11F2-3 heavy chain

    Plasmid

    #198253

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1_ E3_5 _hFc

    Plasmid

    #199617

    Purpose

    Encodes DARPin-hFc E3_5 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc E3_5 (control)
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCDNA.3-V5-SPOP (R221C)

    Plasmid

    #197022

    Purpose

    Mammalian expression vecor for V5-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R221C

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA.3-V5-SPOP (R354H)

    Plasmid

    #197023

    Purpose

    Mammalian expression vecor for V5 tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R354H

    Promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA4/TO-SPOP-myc (WT)

    Plasmid

    #197001

    Purpose

    Mammalian inducible expression construct of myc-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    -

    Promoter

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RCav3.1/pcDNA3-HE3

    Plasmid

    #197324

    Purpose

    Plasmid expressing an antigen targeting the Cav3.1 calcium channel subunit

    Depositor

    Edward Perez-Reyes

    Insert

    Cav3.1 (Cacna1g Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Sur2A flag/pCDNA3

    Plasmid

    #197295

    Purpose

    Plasmid expressing an antigen targeting Sur2A

    Depositor

    William Coetzee

    Insert

    Sur2A (Abcc9 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge