We narrowed to 4,424 results for: ARA-2

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4ΔCTD

Plasmid

#157800

Purpose

Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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BRCA1-(219-408)

Plasmid

#35067

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 219-408

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
AtCOPT2 uPIC

Plasmid

#117900

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

AtCOPT2 uPIC (COPT2 Mustard Weed)

Expression

Yeast

Promoter

AOX

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SLAH2

Plasmid

#117104

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

SLAH2 (SLAH2 Mustard Weed)

Tags

His

Expression

Yeast

Promoter

AOX

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBI/Rabaptin-5

Plasmid

#171851

Purpose

inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

Depositor

Guangpu Li

Article

Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

Insert

Rabaptin-5 (human)

Expression

Mammalian

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hPMLIII

Plasmid

#103824

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-hPMLIII (CRY2, PML Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS-VP16

Plasmid

#103821

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hPMLIII

Plasmid

#103825

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hPMLIII (CRY2, PML Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PEPR2 nD1_pECIA2

Plasmid

#115093

Purpose

Bait vector PEPR2 nD1_pECIA2 should be used with prey vector PEPR2 nD1_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17750 (PEPR2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PEPR2 nD1_pECIA14

Plasmid

#114893

Purpose

Prey vector PEPR2 nD1_pECIA14 should be used with bait vector PEPR2 nD1_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17750 (PEPR2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDD56

Plasmid

#119883

Purpose

Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_EYFP

Expression

Bacterial

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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