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Plasmid#235450PurposeNOT gate receiver with bs for sgRNA2DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.3
Plasmid#235451PurposeNOT gate receiver with bs for sgRNA3DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.4
Plasmid#235452PurposeNOT gate receiver with bs for sgRNA4DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.5
Plasmid#235453PurposeNOT gate receiver with bs for sgRNA5DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.6
Plasmid#235454PurposeNOT gate receiver with bs for sgRNA6DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH896
Plasmid#230840PurposeEf1a-DivA-BE-T2A-tagBFP-wPREDepositorInsertDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH911
Plasmid#230856PurposeEf1a-BE3-T2A-BlastR-wPREDepositorInsertBE3 (rAPOBEC1)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH941
Plasmid#230846PurposeEf1a-DivA-BE-T2A-GFP-wPREDepositorInsertDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH897
Plasmid#230841PurposeEf1a-nDivA-BE-T2A-tagBFP-wPREDepositorInsertnDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH811
Plasmid#230850PurposeEf1a-nDivA-BE-T2A-BlastR-wPREDepositorInsertnDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH915
Plasmid#230843PurposeEf1a-BE3-T2A-tagBFP-wPREDepositorInsertBE3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH810
Plasmid#230849PurposeEf1a-DivA-BE-T2A-BlastR-wPREDepositorInsertDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH720
Plasmid#230845PurposeEf1a-ABE7.10-T2A-tagBFP-wPREDepositorInsertABE7.10
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PT7extended-MGapt-UTR1-deGFP
Plasmid#227646PurposepT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis with extended T7 promoter.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)
Plasmid#225436PurposeBeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.DepositorInsertBeYDV geminino 1.0
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
Plasmid#225430PurposeOCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-FRT-OCSterm-FRT-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEU
Plasmid#213846PurposeLEU integrating plasmid backbone used to create all of the reporter plasmids.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only