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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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CAG_mCh_p85a_mCh

Plasmid

#199630

Purpose

FRET acceptor for IGF1R sensor

Depositor

Ryohei Yasuda

Article

Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

Insert

p85a-SH2 (Pik3r1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pmRuby2-CrowdingLinker-Clover

Plasmid

#171058

Purpose

Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

Crowdedness-sensitive linker domain

Tags

mClover2 and mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJJB1561

Plasmid

#218626

Purpose

Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCZGY847

Plasmid

#135093

Purpose

Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

Depositor

Yishi Jin

Article

Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

Insert

Pacr-2

Tags

mcherry and unc-10 3'UTR

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP-mTLN1-FL

Plasmid

#164838

Purpose

Expression of GFP tagged FL mouse talin1

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Rep78-III/CAG/WPREΔU3

Plasmid

#190727

Purpose

Expression of AAV2 Rep78-III in mammalian cells

Depositor

Jakob Reiser

Article

Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

Insert

AAV2 Rep78-III

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo2

Plasmid

#25102

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO2 (SUMO2 Human)

Tags

His

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pmTFP1-ShadowG

Plasmid

#99867

Purpose

Measure FRET between mTFP1 and ShadowG

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

ShadowG

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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APT3-PTEN (PTEN high)

Plasmid

#168006

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T3'PAP

Use

Synthetic Biology

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-BoNT/E

Plasmid

#168020

Purpose

Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/E-LC(1-411)-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-BlastR

Plasmid

#220967

Purpose

Expresses human codon-optimized SauriCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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APT3-VAMP4

Plasmid

#168004

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP4(I75-L91)-T3'PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-E(4130)A2

Plasmid

#168021

Purpose

Expresses E(4130)A2-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

E(4130)A2-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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LLhG-pHG165a E62K

Plasmid

#90051

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

Insert

LLhG/E62K

Expression

Bacterial

Mutation

E62K

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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