Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,256 results for: D10

Clear keywords
Showing: 1801 - 1820 of 2256 results

  5. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

    Enlarge

  8. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

    Enlarge

  9. TFORF0724

    Plasmid
    #143070
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143070-plasmid-map-sequence-id-398863

    Enlarge

  20. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Article
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    131124-plasmid-map-sequence-id-257454

    Enlarge

Showing: 1801 - 1820 of 2256 results