We narrowed to 2,231 results for: D10

1. pRS0045

   Plasmid

   #131124

   Purpose

   Mammalian Target-ACE expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1, TadA, and UGI

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

   Plasmid

   #163554

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-STIM1-NN_neomycin

   Plasmid

   #65311

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniCGBE1-VRQR (pBM1173)

   Plasmid

   #140255

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_VRQR-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-BE4GamRA-P2A-Puro

   Plasmid

   #112673

   Purpose

   Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE4GamRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-CAD

   Plasmid

   #73566

   Purpose

   Soluble red fluorescent activator of Orai1 channels

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   mCherry-CAD (STIM1 342-448) (STIM1 Human, Synthetic)
   

   Tags

   6x His and mCherry

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

    Plasmid

    #134611

    Purpose

    Mammalian expression of mouse KIF5A 378-1027

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX_305-N-dTAG_OGT

    Plasmid

    #155196

    Purpose

    N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    lacking N-terminal Met

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR1A mKate2-hu_ncOGT WT

    Plasmid

    #154282

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    WT OGT

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-gfaABC1D-monSTIM1

    Plasmid

    #161797

    Purpose

    Lentiviral vector expressing monSTIM1 in astrocytes.

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    gfaABC1D

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Human, Budding Yeast, Synthetic)
    

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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