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pMT-Aurora B-GFP

Plasmid

#24285

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Aurora B Kinase (aurB Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK1

Plasmid

#197108

Purpose

Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (TAOK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Bgeo

Plasmid

#13837

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Expression

Mammalian

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
Mito-meCpG

Plasmid

#186462

Purpose

Expresses GFP tagged methyl-CpG recognition motif targeted to mitochondria in mammalian cells.

Depositor

Chongli Yuan

Article

Zhao et al ACS Sens. 2021 Oct 22;6(10):3575-3586. d

Insert

dimeric MBD1 (MBD1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVpic2blast-EGFP-FF2

Plasmid

#164923

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Hyg

Plasmid

#13836

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Expression

Mammalian

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1318

Plasmid

#78587

Purpose

Expression flag-USP19 494-1318 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1318 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pMX-eSOX17WHC

Plasmid

#118839

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17WHC (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

WHC: L46W, V53H, E57C

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Bex2

Plasmid

#131607

Purpose

expression of mouse Bex2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Bex2 (Bex2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only