159,371 results

1. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Use

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

   Plasmid

   #164822

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   miniCMV and pGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

   Plasmid

   #227246

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFR-mApple

   Plasmid

   #203773

   Purpose

   Number and brightness analysis of EGFR oligomerization

   Depositor

   Thorsten Wohland

   Article

   Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6a:sgRNA#2

   Plasmid

   #64246

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ114

   Plasmid

   #85995

   Purpose

   One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified bovine U6-2

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIRECT_22A

   Plasmid

   #91133

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C2-GGA2

   Plasmid

   #199172

   Purpose

   Expresses GFP-GGA2 fusion in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2 (GGA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Ala 23 Thr substitution; silent substitution in c…

   Promoter

   CMV

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB1-nbOcIgG-Tn5

    Plasmid

    #184285

    Purpose

    Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbOcIgG-Tn5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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