We narrowed to 13,309 results for: sequence
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Plasmid#166541PurposescFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184739Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK7a1-EE
Plasmid#47578PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1DepositorInsertMKK7a1-EE (Map2k7 Mouse)
Tags3xHA and GSTExpressionBacterialMutationResidues 73-420 of RefSeq sequence. Ser271 to Gl…Available SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hiv7CMV-Cremyc-2A-GFP
Plasmid#117148PurposeExpress Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.DepositorInsertCremyc-2A-GFP
UseLentiviralAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1.1-Tr2-eGFP
Plasmid#162782PurposeFluorescent reporter for protein expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(L)-AP-His
Plasmid#72017PurposeExpresses the Sema3D protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Golgi-7
Plasmid#55486PurposeLocalization: GalT Targeting Sequence, Excitation: 462, Emission: 492DepositorInsertGolgi (B4GALT1 Human)
TagsmTFP1ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
P41-bio
Plasmid#47739PurposeExpresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P41
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Plxnb3-Fc-His
Plasmid#72130PurposeExpresses the extracellular region of the PlexinB3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB4-3
Plasmid#121536PurposesgITGB4-3 sequence: GAGGAGCGTAGGTCCTCGCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB4-3
UseCRISPR and LentiviralTagsmCherryExpressionMammalianPromotermouse U6Available SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only