We narrowed to 5,868 results for: nls
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Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2
Plasmid#182347PurposeGFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65
Plasmid#104566PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domainDepositorInsertdLbCpf1(D832A)-p65
UseTags3x HA and NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)
Plasmid#114091PurposeCAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a (E174R/S542R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R and S542RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH
Plasmid#112211PurposeTargeted DNA methylationDepositorInsertC-terminus of dCas9
UseAAVTagsExpressionMutationD10APromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2
Plasmid#182349PurposemScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
Plasmid#159795PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertpEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP
Plasmid#226113PurposeMammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.DepositorInsertAcrIIC3
UseTagsFLAG, hsa-miR-1-5p Target Sites, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP
Plasmid#226111PurposeMammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.DepositorInsertAcrIIC3
UseTagsFLAG, hsa-miR-22 Target Sites, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)
Plasmid#229827PurposeA CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcriptsDepositorInsert3xnls-TagBFP2-MCS-FLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)
Plasmid#229838PurposeA Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.DepositorInsertLexA-mODC-LexAOp
UseTol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls BFP FLAG WPRE bGH pA (JDW 484)
Plasmid#224539PurposeA Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleusDepositorInsertnls-mTagBFP2-FLAG_WPRE-bGH-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
Plasmid#220613PurposeCre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.DepositorInsertFLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
Plasmid#220609PurposeNeuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.DepositorInsertFLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianMutationPromoterhSynAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222857PurposeThis plasmid codes for the Slug-KH nickase with a sg(CTG)6DepositorInsertSlug-KHD10A+sgCTG
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222859PurposeThis plasmid codes for the Slug-KH nickase with a sg(AGC)6DepositorInsertSlug-KHD10A+sgAGC
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222858PurposeThis plasmid codes for the Slug-KH nickase with a sg(CAG)6DepositorInsertSlug-KHD10A+sgCAG
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222860PurposeThis plasmid codes for the Slug-KH nickase with a sg(GCA)6DepositorInsertSlug-KHD10A+sgGCA
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)
Plasmid#224524PurposeA Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassetteDepositorInsertrox-3xNLS-mCherry-V5-2xSTOP-rox
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet
Plasmid#220614PurposeCre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only