We narrowed to 2,426 results for: 683

1. pGL3-Basic-Fascin-Promoter (-402-0)

   Plasmid

   #89823

   Purpose

   Promoter luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRKD3 gRNA (BRDN0001146689)

   Plasmid

   #75951

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD3 (PRKD3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRKD3 gRNA (BRDN0001146945)

   Plasmid

   #75953

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD3 (PRKD3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Luc-Hdm4-3'UTR-F2d5

   Plasmid

   #64018

   Purpose

   Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

   Depositor

   Lin He

   Article

   Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

   Insert

   HDMX (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_MIA3-1/1

   Plasmid

   #91327

   Purpose

   Protein expression and purification of human SH3 domain construct MIA3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   MIA3-1/1 (MIA3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3b(1454/3172)P2P-mut

   Plasmid

   #21405

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   hypoxia response element mutated

   Promoter

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

   Plasmid

   #51390

   Depositor

   Narry Kim

   Article

   Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

   Insert

   NM_001039802.1 (CDC42 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   miR-29 binding site mutant (position 577-599, 997…

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-Cerulean-UCR1

   Plasmid

   #67578

   Purpose

   Expresses the UCR1 region of PDE4D3 in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

   Insert

   Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
   

   Use

   Tags

   Cerulean and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid residues 17-136 of PDE4D3

   Promoter

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pOP795

   Plasmid

   #235700

   Purpose

   Protein expression His-taged HsNRBF2_FL in bacteria

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Insert

   NRBF2 (NRBF2 Human)
   

   Use

   Tags

   His6(TEV)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOP796

    Plasmid

    #235701

    Purpose

    Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 MIT domain (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    aa 1-89

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A 2xHA GPR137

    Plasmid

    #226809

    Purpose

    Gateway entry vector for GPR137

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_001164351

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-actin

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ A-SLC-His+Strep-Opa1 (SB254)

    Plasmid

    #227603

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    Opa1
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-960 only

    Promoter

    AOX1

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

    Plasmid

    #227606

    Purpose

    Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    R247A,R347A

    Promoter

    AOX1

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LBR(R583Q)-FKBP-GFP

    Plasmid

    #222423

    Purpose

    To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(R583Q) (LBR Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    R583Q

    Promoter

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LBR(N547D)-FKBP-GFP

    Plasmid

    #222422

    Purpose

    To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(N547D) (LBR Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    N547D

    Promoter

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GPR6-DuET

    Plasmid

    #213286

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR6 (GPR6 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1 myc-Rab10-mito

    Plasmid

    #208367

    Purpose

    Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10-mito (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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