We narrowed to 1,878 results for: Ipp

1. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

   Plasmid

   #225440

   Purpose

   Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.

   Depositor

   Diego Orzaez

   Insert

   nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28:LanIV-Chondromyces

   Plasmid

   #225078

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIV-Chondromyces)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28:LanI-101

   Plasmid

   #225065

   Purpose

   Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanI-101)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-cFOS-FRB-VP16-P2A-EGFP

   Plasmid

   #210510

   Purpose

   expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   FRB-VP16-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cFos

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LR (hsp70I: cPLA2-mK2)

   Plasmid

   #162564

   Purpose

   Measuring cPLA2 activation in live cells after heat shock.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Inserts

   Heat shock protein 70 (HSPA1A Human)
   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Gateway donor vector

   Tags

   mK2

   Expression

   Mutation

   Promoter

   cmlc2

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFL His-I3

   Plasmid

   #113513

   Purpose

   Expression of His-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMGF196

   Plasmid

   #97005

   Purpose

   Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMGF182

   Plasmid

   #97006

   Purpose

   Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   CHMP7 (CHMP7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR I-Ab beta Derp1-117

    Plasmid

    #113639

    Purpose

    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    

    Use

    Tags

    6x His tag, fused to Der p 1 (117-127) peptide vi…

    Expression

    Insect

    Mutation

    transmembrane and cytoplasmic domains truncated

    Promoter

    Metallothionein

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SUPERBLADE5

    Plasmid

    #134913

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHES839

    Plasmid

    #87941

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. H11-mScarletSIIN

    Plasmid

    #172438

    Purpose

    Hipp11 targeting vector, expresses mScarlet-SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    mScarlet-SIIN
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SET

    Plasmid

    #234708

    Purpose

    expresses His-tagged human SET in E coli

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

    Plasmid

    #162566

    Purpose

    Used for the dual color labeling of cPLA2 and LaminB1 expression.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Lamin B1 (lmnb1 Zebrafish)
    

    Use

    Tags

    EGFP, P2A, and mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB1N-LZ-LOV2(wt)

    Plasmid

    #107614

    Purpose

    N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHES795

    Plasmid

    #87943

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1N-mScarlet-LZ-LOV2

    Plasmid

    #197419

    Purpose

    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H11-mScarletALG8

    Plasmid

    #172440

    Purpose

    Hipp11 targeting vector, expresses mScarlet-mALG8

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    mScarlet-mALG8
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge