We narrowed to 2,278 results for: Tub;

1. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

   Plasmid

   #191331

   Purpose

   Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

   Plasmid

   #182887

   Purpose

   Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   silet mutations in aa 197 - 203 to confer resista…

   Promoter

   EF1alpha core

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

   Plasmid

   #182885

   Purpose

   Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   stop codon introduced 2 amino acids downstream of…

   Promoter

   EF1alpha core

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

   Plasmid

   #180314

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 )
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   SEPT9_i1-i5

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_SEPT9_i1 S22A S23A-msfGFP

   Plasmid

   #180338

   Purpose

   mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S22A S23A

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_SEPT9_i1 R10A R15A-msfGFP

   Plasmid

   #180336

   Purpose

   mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R10A R15A

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_SEPT9_i1 S12A S13A-msfGFP

   Plasmid

   #180337

   Purpose

   mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S12A S13A

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SITS-EGFP-Bin1b

   Plasmid

   #176021

   Purpose

   Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Bin1b (bin1b Zebrafish)
   

   Use

   Leishmania cell free expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cavin4a-mCherry

   Plasmid

   #176010

   Purpose

   Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Cavin4a (cavin4a Zebrafish)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

    Plasmid

    #108503

    Purpose

    inducible expression of vsv-INCENP d543-746-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746 (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha helix

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP

    Plasmid

    #108505

    Purpose

    inducible expression of vsv-INCENP 8KR-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    K548R, K563R, K602R, K609R, K614R, K622R, K630R, …

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP

    Plasmid

    #108506

    Purpose

    inducible expression of vsv-INCENP 8KQ-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

    Plasmid

    #70667

    Purpose

    Human mutant of E2F1 lacking amino acids 193-359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 193 to 359 (E2F1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacks amino acids 193-359

    Promoter

    cmv

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

    Plasmid

    #70669

    Purpose

    Human mutant of E2F1 lacking amino acids 284 to 359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 284 to 359 (E2F1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks amin acids 284 to 359

    Promoter

    cmv

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET21a-MKP7_K62R_153

    Plasmid

    #37795

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 62 to Arginine and deleted amino a…

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET21a-MKP7_K52R_153

    Plasmid

    #37793

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 52 to Arginine and deleted amino a…

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-TetON-Sox2

    Plasmid

    #110280

    Purpose

    Doxycycline-inducible overexpression of Sox2

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    No fusion genes

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    TRE

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOmnibac_ZZ_linker_K963_GFP_SNAP

    Plasmid

    #159727

    Purpose

    Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
    

    Use

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid

    #69113

    Purpose

    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Silent mutations to confer shRNA resistance.

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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