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pNLF1-C_hCOL4A5-Nluc_S1661X

Plasmid

#198123

Purpose

Express hCOL4A5_S1661X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1661X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmGamillus

Plasmid

#182971

Purpose

Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmGamillus

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM L368G

Plasmid

#169985

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, L368G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM R394E

Plasmid

#169987

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, R394E

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F405G

Plasmid

#169988

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F405G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F370G

Plasmid

#169986

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G + S297A

Plasmid

#169974

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R + S297A

Plasmid

#169968

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E + S297A

Plasmid

#169972

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM E274R

Plasmid

#169977

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 T755N

Plasmid

#166820

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

T755N

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 R726W

Plasmid

#166819

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

R726W

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHTSHP-ShhN-Cys

Plasmid

#121136

Purpose

Expresses ShhN with a C-terminal cysteine residues in bacteria

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

shh (Shh Mouse)

Tags

HIS, HRV3C site, and MBP

Expression

Bacterial

Mutation

Amino acid sequence from 26-191, with an addition…

Promoter

tac

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-wt

Plasmid

#67909

Purpose

Inducible expression of PIAL2 in E.coli

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

Plasmid

#58490

Purpose

Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Glutamine

Promoter

Ubiquitin

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

Plasmid

#104567

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain

Use

CRISPR

Tags

NLS-3xHA-VPR

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

Plasmid

#205444

Purpose

human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-HLA-E SCT-hCD55

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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