165,688 results

1. pCENPTΔC-dCas9-3xGFP

   Plasmid

   #198325

   Purpose

   Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   CENPTΔC (CENPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP x 3 and dCas9

   Expression

   Mammalian

   Mutation

   truncation - encodes only amino acids 1-375

   Promoter

   CMV-TetO

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1 hMOG-alpha1-EGFP

   Plasmid

   #126463

   Purpose

   Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Myelin oligodendrocyte glycoprotein (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPT24-mtnluc

   Plasmid

   #184339

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Insert

   nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
   

   Expression

   Yeast

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1761

   Plasmid

   #142843

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF24 (ZNF24 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. B1-INTEGRIN WT

   Plasmid

   #195215

   Purpose

   Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

   Depositor

   David Bryant, Valerie Weaver

   Article

   Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Expression

   Mammalian

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJH46

   Plasmid

   #67272

   Purpose

   Conjugally transferable red recombinogenic plasmid

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   traJ, cat, oriT
   

   Use

   Red recombinase

   Promoter

   cat gene promoter from PGNS-BAC

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p4455 FLAG-hPLIC-2

   Plasmid

   #8661

   Depositor

   Peter Howley

   Article

   Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

   Insert

   hPLIC-2 (UBQLN2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Neo-Bam p53 R273H

   Plasmid

   #16439

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R273H (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R273H

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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