Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,626 results for: GFP expression plasmids

Clear keywords
Showing: 4601 - 4650 of 6626 results

  6. pT7-EGFP-C1-HsXRN1_1-1596_O

    Plasmid
    #147092
    Purpose
    Mammalian Expression of HsXRN1_1-1596
    Depositor
    Elisa Izaurralde
    Insert
    HsXRN1_1-1596 (XRN1 Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    147092-plasmid-map-sequence-id-355414

    Enlarge

  7. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

    Enlarge

  11. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

    Plasmid
    #172200
    Purpose
    AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172200-plasmid-map-sequence-id-338847

    Enlarge

  12. pAc5.1B-EGFP-DmBelle_1-298_B

    Plasmid
    #145913
    Purpose
    Insect Expression of DmBelle_1-298
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle_1-298 (bel Fly)
    Expression
    Insect
    Mutation
    del 158-160 (GGG)
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    145913-plasmid-map-sequence-id-333036

    Enlarge

  17. AAV-∆Hapln1

    Plasmid
    #200778
    Purpose
    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter
    Depositor
    Alexander Dityatev
    Article
    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d
    Has Service
    AAV8
    Insert
    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    Use
    AAV
    Tags
    cfGFP
    Mutation
    deleted lectican binding domain (aminoacids 40-15…
    Promoter
    Synapsin
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200778-plasmid-map-sequence-id-394043

    Enlarge

  19. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

    Enlarge

  21. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid
    #208292
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpCas9(D10A)
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208292-plasmid-map-sequence-id-410847

    Enlarge

  22. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

    Enlarge

  23. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

    Enlarge

  24. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

    Enlarge

  30. YIplac211-IMH1(-GRIP)-msGFP2-FRBx2-HA

    Plasmid
    #255058
    Purpose
    Remove the Imh1 GRIP domain sequence and insert a C-terminal msGFP2-FRBx2-HA tag at IMH1 by popin/popout.
    Depositor
    Benjamin Glick
    Insert
    Imh1(-GRIP)-msGFP2-FRBx2-HA
    Expression
    Yeast
    Mutation
    Imh1 GRIP domain removed
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    255058-plasmid-map-sequence-id-511750

    Enlarge

  31. pAAV-synmiR-L-mRuby-Pro-EGFP-4×19

    Plasmid
    #218264
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×19
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    218264-plasmid-map-sequence-id-509434

    Enlarge

  32. pAAV-synmiR-7-mRuby-Pro-EGFP-8×12

    Plasmid
    #218253
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR7-mRuby-EF1a-CMVe_MeP-EGFP-MRE7_8×12
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    218253-plasmid-map-sequence-id-509430

    Enlarge

  33. pAAV-synmiR-5-mRuby-Pro-EGFP-4×9

    Plasmid
    #218241
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×9
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    218241-plasmid-map-sequence-id-498517

    Enlarge

  34. pAAV-synmiR-7-mRuby-Pro-EGFP-4×12

    Plasmid
    #218252
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR7-mRuby-EF1a-CMVe_MeP-EGFP-MRE7_4×12
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    218252-plasmid-map-sequence-id-498516

    Enlarge

  37. pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA

    Plasmid
    #223546
    Purpose
    Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene
    Depositor
    Sally Lowell
    Article
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, mCherry-3NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223546-plasmid-map-sequence-id-442169

    Enlarge

  40. pAAV-synmiR-10-mRuby-Pro-EGFP-8×8

    Plasmid
    #218260
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218260-plasmid-map-sequence-id-494333

    Enlarge

  41. pAAV-synmiR-10-mRuby-Pro-EGFP-8×9

    Plasmid
    #218261
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×9
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218261-plasmid-map-sequence-id-494523

    Enlarge

  42. pAAV-synmiR-4-mRuby-Pro-EGFP-4×8

    Plasmid
    #218237
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_4×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218237-plasmid-map-sequence-id-494335

    Enlarge

  43. pAAV-synmiR-4-mRuby-Pro-EGFP-8×8

    Plasmid
    #218238
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218238-plasmid-map-sequence-id-494305

    Enlarge

  44. pAAV-synmiR-5-mRuby-Pro-EGFP-4×8

    Plasmid
    #218240
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218240-plasmid-map-sequence-id-494332

    Enlarge

  45. pAAV-synmiR-5-mRuby-Pro-EGFP-4×12

    Plasmid
    #218242
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×12
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218242-plasmid-map-sequence-id-494327

    Enlarge

  46. pAAV-synmiR-5-mRuby-Pro-EGFP-8×8

    Plasmid
    #218243
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218243-plasmid-map-sequence-id-494518

    Enlarge

  47. pAAV-synmiR-8-mRuby-Pro-EGFP-4×8

    Plasmid
    #218254
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR8-mRuby-EF1a-CMVe_MeP-EGFP-MRE8_4×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218254-plasmid-map-sequence-id-494306

    Enlarge

  48. pAAV-synmiR-8-mRuby-Pro-EGFP-4×9

    Plasmid
    #218255
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR8-mRuby-EF1a-CMVe_MeP-EGFP-MRE8_4×9
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218255-plasmid-map-sequence-id-494313

    Enlarge

  49. pAAV-synmiR-8-mRuby-Pro-EGFP-8×8

    Plasmid
    #218256
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR8-mRuby-EF1a-CMVe_MeP-EGFP-MRE8_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218256-plasmid-map-sequence-id-494515

    Enlarge

  50. pAAV-synmiR-9-mRuby-Pro-EGFP-4×16

    Plasmid
    #218257
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR9-mRuby-EF1a-CMVe_MeP-EGFP-MRE9_4×16
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218257-plasmid-map-sequence-id-494312

    Enlarge

Showing: 4601 - 4650 of 6626 results