We narrowed to 5,495 results for: 129

1. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TetR-tdTomato

   Plasmid

   #212958

   Purpose

   piggyBac donor vector containing TetR-tdTomato for random integration by PBase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   TetR-tdTomato
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112922

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR1446

   Plasmid

   #111129

   Purpose

   Expresses FANCF

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCF (FANCF Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-NES-mTagBFP2

   Plasmid

   #216129

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-FKBP-TMPRSS3

   Plasmid

   #221298

   Purpose

   Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TMPRSS3 (pLxIS) (Tmprss3 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PUM1_PUM_pGEX

   Plasmid

   #135129

   Purpose

   Recombinant expression of PUM1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PUM1 (PUM1 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mNRDc-V5

    Plasmid

    #51297

    Purpose

    expression of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc (Nrd1 Mouse)
    

    Use

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pL0_AC-CEN_ARS_HIS

    Plasmid

    #202129

    Purpose

    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    CEN_ARS_HIS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ARS sequence mutated to remove sapI site for dome…

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

    Plasmid

    #112921

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hoxb7 promoter

    Plasmid

    #21294

    Depositor

    Frank Costantini

    Article

    Srinivas et al Development. 1999 Apr . 126(7):1375-86.

    Insert

    Hoxb7 promoter (Hoxb7 Mouse)
    

    Use

    Promoter can be used to make transgenic mice

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pYSD153

    Plasmid

    #212929

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYSD141

    Plasmid

    #212928

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_SH3D21-1/3

    Plasmid

    #91296

    Purpose

    Protein expression and purification of human SH3 domain construct SH3D21-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3D21-1/3 (SH3D21 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_MYO15A-2/2

    Plasmid

    #91291

    Purpose

    Protein expression and purification of human SH3 domain construct MYO15A-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO15A-2/2 (MYO15A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_SPTAN1-1/1

    Plasmid

    #91292

    Purpose

    Protein expression and purification of human SH3 domain construct SPTAN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SPTAN1-1/1 (SPTAN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL1

    Plasmid

    #184129

    Purpose

    For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Pmrp-1 (3kb)

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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