We narrowed to 9,766 results for: 158

1. pDONR221 Ub-NLRP1 S1213 P1214R

   Plasmid

   #166830

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 Ub-NLRP1 S1213

   Plasmid

   #166827

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 NLRP1 T755N

   Plasmid

   #166820

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   T755N

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 NLRP1 R726W

   Plasmid

   #166819

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   R726W

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 NLRP1 FIIND (F1079M-L1373)

   Plasmid

   #166831

   Purpose

   Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKM59

   Plasmid

   #154158

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Enhancer for the Drosophila melanogaster Metchnik…

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28P_dCrk

   Plasmid

   #131145

   Purpose

   Expresses 6XHis::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPR sgAMDHD1

   Plasmid

   #102316

   Purpose

   genetic depletion of AMDHD1

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgAMDHD1 (AMDHD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sema4f.a-Fc-His

    Plasmid

    #72158

    Purpose

    Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4f.a (Sema4f Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE ENTR CMV iRFP R4-R3

    Plasmid

    #62158

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    iRFP
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX_305-N-dTAG

    Plasmid

    #91797

    Purpose

    Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A-nBRD4/PITCh

    Plasmid

    #91794

    Purpose

    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EXT1-pLJC2-3XFLAG

    Plasmid

    #215818

    Purpose

    Ectopic expression of EXT-1 in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307-CRBN-3F-BirA

    Plasmid

    #220146

    Purpose

    Expresses CRBN-BirA for CRBN substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSynapsin-PE2-Cterminal-lenti

    Plasmid

    #135956

    Purpose

    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Prime editor v2 (PE2) C-terminal
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MMLV RT(D200N, T306K, W313F, T330P, L603W)

    Promoter

    hSynapsin

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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