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p663-UBC-miniTurbo-V5-PIP4K2C_IDG-K

Plasmid

#170598

Purpose

Gateway destination clone of PIP4K2C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PIP4K2C (PIP4K2C Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 2, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-TSSK3_IDG-K

Plasmid

#170617

Purpose

Gateway destination clone of TSSK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-TSSK3 (TSSK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-TESK1_IDG-K

Plasmid

#170614

Purpose

Gateway destination clone of TESK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-TESK1 (TESK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-STK40_IDG-K

Plasmid

#170613

Purpose

Gateway destination clone of STK40 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-STK40 (STK40 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-STK38L_IDG-K

Plasmid

#170612

Purpose

Gateway destination clone of STK38L (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-STK38L (STK38L Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-RPS6KL1_IDG-K

Plasmid

#170605

Purpose

Gateway destination clone of RPS6KL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-RPS6KL1 (RPS6KL1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PRKACG_IDG-K

Plasmid

#170603

Purpose

Gateway destination clone of PRKACG (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PRKACG (PRKACG Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PHKG1-V5-miniTurbo_IDG-K

Plasmid

#158192

Purpose

Gateway destination clone of PHKG1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PHKG1-V5-miniTurbo (PHKG1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-TLK2-V5-miniTurbo_IDG-K

Plasmid

#135249

Purpose

Gateway destination clone of TLK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TLK2-V5-miniTurbo (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSKAP_AX

Plasmid

#122726

Purpose

pSKAP plasmid containing the unique DNA barcode "AX" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AX

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AW

Plasmid

#122725

Purpose

pSKAP plasmid containing the unique DNA barcode "AW" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AV

Plasmid

#122724

Purpose

pSKAP plasmid containing the unique DNA barcode "AV" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AQ

Plasmid

#122719

Purpose

pSKAP plasmid containing the unique DNA barcode "AQ" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AQ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AP

Plasmid

#122718

Purpose

pSKAP plasmid containing the unique DNA barcode "AP" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AM

Plasmid

#122715

Purpose

pSKAP plasmid containing the unique DNA barcode "AM" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AL

Plasmid

#122714

Purpose

pSKAP plasmid containing the unique DNA barcode "AL" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AK

Plasmid

#122713

Purpose

pSKAP plasmid containing the unique DNA barcode "AK" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AJ

Plasmid

#122712

Purpose

pSKAP plasmid containing the unique DNA barcode "AJ" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AJ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AF

Plasmid

#122708

Purpose

pSKAP plasmid containing the unique DNA barcode "AF" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge