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Showing: 1841 - 1860 of 4039 results

  1. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cGR2176
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188258-plasmid-map-sequence-id-377988

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  4. pICE-EGFP-FLAG-Ku70siR-WT

    Plasmid
    #46961
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    human Ku70 (XRCC6 Human)
    Use
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Tet
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46961-plasmid-map-sequence-id-66851

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  13. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1841 - 1860 of 4039 results