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pDONR223_MTM1_WT

Plasmid

#81908

Purpose

Gateway Donor vector containing MTM1 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E410K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.D194Y

Plasmid

#81401

Purpose

Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D194Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-Flag-FBW7K404R

Plasmid

#200717

Purpose

The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

FBW7 K404 is mutated to Alanine.

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SDHA_WT

Plasmid

#82278

Purpose

Gateway Donor vector containing SDHA, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SDHA (SDHA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNF_WT

Plasmid

#81824

Purpose

Gateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNF (TNF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.D176H

Plasmid

#82870

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D176H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G251F

Plasmid

#82862

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G251F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G56V

Plasmid

#82760

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G56V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G242W

Plasmid

#82763

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G242W

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G251V

Plasmid

#82765

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G251V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.L245F

Plasmid

#82751

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L245F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_YY1_WT

Plasmid

#82171

Purpose

Gateway Donor vector containing YY1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YY1 (YY1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PYGL_WT_V5

Plasmid

#82991

Purpose

Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PYGL (PYGL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CCNT1_pLX307

Plasmid

#98328

Purpose

Lentiviral expression of CCNT1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CCNT1 (CCNT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

H519R, S566P, and P712S

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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ADSL_pLX307

Plasmid

#98311

Purpose

Lentiviral expression of ADSL

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ADSL (ADSL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-GST-FBW7 alpha

Plasmid

#197456

Purpose

The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

In vitro translation

Tags

GST

Expression

Bacterial

Mutation

See depositor comments below

Promoter

T7

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CD_WT

Plasmid

#82222

Purpose

Gateway Donor vector containing PIK3CD , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CD (PIK3CD Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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