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pMiR-RECK-3'UTR-miR-7-mut

Plasmid

#53692

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Tags

Expression

Mutation

nucleotide position 171-174 are mutated to GAAG

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Seed Mut

Plasmid

#32490

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)

Plasmid

#15384

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS123…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS1

Plasmid

#16566

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS2

Plasmid

#16567

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS2

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBV-Luc mut MBS4

Plasmid

#16570

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS4

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pXJ-CD274-Nluc

Plasmid

#232658

Purpose

Nluc-tagged PD-L1 expresion in mammalian cells; lectin-captured luciferase reporter assay

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Nluc-_(PLRSKK)-CA1-19

Plasmid

#232657

Purpose

Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CGA (CGA Human)

Use

Luciferase

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_04

Plasmid

#158105

Purpose

CaV1.3 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1D (uc003dgu.5)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_03

Plasmid

#158104

Purpose

CaV1.2 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1C (uc009zdu.1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-TAK1 5'-UTR

Plasmid

#115356

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

TAK1 5'-UTR (MAP3K7 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-210-0)

Plasmid

#89826

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-IRES-miR34a-P6

Plasmid

#64790

Purpose

Luciferase expression driven by miR-34a promoter P6

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P6 (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P3

Plasmid

#64787

Purpose

Luciferase expression driven by miR-34a promoter P3

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P3 (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P5

Plasmid

#64789

Purpose

Luciferase expression driven by miR-34a promoter P5

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P5 (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P4

Plasmid

#64788

Purpose

Luciferase expression driven by miR-34a promoter P4

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P4 (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K2S

Plasmid

#62980

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 446-911 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only