We narrowed to 2,540 results for: organization Addgene

1. Lenti-mApple-6xHp

   Plasmid

   #217376

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   mApple-6x Hp NC
   

   Use

   Lentiviral

   Tags

   mApple

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FAB(ER-LD)

   Plasmid

   #214424

   Purpose

   Detection of ER-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FRB-V5
   

   Tags

   CFAST-Halo and RspAN-FRB-V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CFAST-Halotag-ER

   Plasmid

   #214413

   Purpose

   ER localization of CFAST fused to Halotag-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   rat cb5 ERM
   

   Tags

   CFAST-Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p7274 MSCV-P C-FlagHA 16 ∆DLYC

   Plasmid

   #163304

   Purpose

   Expresses HPV16 E7 ∆DLYC

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   HPV16 E7 ∆DLYC
   

   Use

   Retroviral

   Tags

   FlagHA

   Mutation

   ∆DLYC

   Promoter

   MSCV LTR

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NFAST(low)-mApple-6xHp

   Plasmid

   #214412

   Purpose

   Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Tags

   mApple-6xHp

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NFAST(low)-Halo-ER

   Plasmid

   #214419

   Purpose

   ER localization of low-affinity NFAST fused to Halo-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p8115 LentiCRISPR v2 sgPTPN14-3

   Plasmid

   #163314

   Purpose

   Expresses Cas9 and a human PTPN14-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgPTPN14-3
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459-TDP 43 sgRNA2

    Plasmid

    #133763

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_WT-talin1 (1-2541)

    Plasmid

    #166112

    Purpose

    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    WT-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Calnexin-HT8-mGold

    Plasmid

    #183989

    Purpose

    Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Tags

    HaloTag8 (C61S, C262S), mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid

    #127362

    Purpose

    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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